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请将下面这句话按照学术英语的表达方式翻译：上清指丁酸梭菌在RCM培养基中培养48H并过滤除菌得到的上清

Answer

The supernatant refers to the supernatant obtained by culturing Clostridium butyricum in RCM medium for 48 hours and filtering for sterilization.

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